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  • 产品名称：CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞

  • 产品型号：CCL-35
  • 产品厂商：美国标准生物品收藏中心（ATCC）
  • 产品价格：0
  • 折扣价格：0
  • 产品文档：
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简单介绍：
CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
详情介绍：
CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞
ATCC® Number: CCL-35?    Price: $438.00
Designations: Pt K1 (NBL-3) [PTK1]
Depositors:  KH Walen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Potorous tridactylis (potoroo)
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Virus Resistance: poliovirus 2
Age:
Gender: female
Comments: The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References: 26188: . . Nature 194: 406, 1962.
26189: . . Cytogenetics 3: 19, 1964.
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