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  • 产品名称： PT-K75 猪鼻甲黏膜成纤维细胞

  • 产品型号：CRL-2528
  • 产品厂商：美国标准生物品收藏中心（ATCC）
  • 产品价格：0
  • 折扣价格：0
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简单介绍：
CRL-2528 PT-K75 猪鼻甲黏膜成纤维细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和*优培养条件！
详情介绍：
CRL-2528 PT-K75 猪鼻甲黏膜成纤维细胞
ATCC® Number: CRL-2528?    Price:  
Designations: PT-K75
Depositors:  CL Kanitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Sus scrofa (pig)
Morphology: fibroblast
Source: Organ: nasal turbinate
Tissue: mucosa
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Virus Susceptibility: Porcine cytomegalovirus
Age: newborn
Comments: The PT-K75 cell line was derived from the turbinate mucosa of two newborn piglets.
The cells are used for the propagation of porcine cytomegalovirus (CMV) by co-cultivation 1:1 with porcine (CMV) infected cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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