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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. It is important to subculture the cells as soon as they become confluent, or else the cells will begin to die.1. Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Do not attempt to break up cell clumpsAdd appropriate aliquots of the cell suspension to new culture vessels. Subcultivation Ratio: 1:2 to 1:6.Incubate cultures at 37°C. Medium Renewal: 1 to 2 times per week
References:
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26004: Wolf K, et al. Duck viral enteritis: microtiter plate isolation and neutralization test using the duck embryo fibroblast cell line. Avian Dis. 18: 427-434, 1974. PubMed: 4368600
26005: Buynak EB, et al. Preparation and testing of duck embryo cell culture rubella vaccine. Am. J. Dis. Child. 118: 347-354, 1969. PubMed: 4307520
53245: Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741
