|
UMR-106 |
|
AE Bogden |
| Biosafety Level: |
1 |
|
frozen |
| Medium & Serum: |
See Propagation |
|
adherent |
|
Rattus norvegicus deposited as Rattus sp. |
|
epithelial
|
|
Organ: bone
Strain: Sprague-Dawley
Disease: osteosarcoma |
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
|
parathyroid hormone (PTH); 1-25(OH)2D3 (bone resorbing steroid hormone) |
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
|
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. |
|
culture medium 95%; DMSO, 5% |
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020 |
|
21441: Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513
21887: . Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977.
22331: Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists and biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768
22684: Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521
22771: Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375
22847: Ingleton PM, et al. Alkaline phosphatase in serum and tumour of rats bearing a hormone- responsive transplantable osteogenic sarcoma. Eur. J. Cancer 15: 685-691, 1979. PubMed: 292595
22873: Crawford A, et al. Membranes from a transplantable osteogenic sarcoma responsive to parathyroid hormone and prostaglandins: regulation of adenylate cyclase and of hormone metabolism. J. Endocrinol. 77: 213-224, 1978. PubMed: 275436
22874: Atkins D, Martin TJ. Rat osteogenic sarcoma cells:effects of some prostaglandins, their metabolites and analogues on cyclic AMP production. Prostaglandins 13: 861-871, 1977. PubMed: 194286
22898: Martin TJ, et al. Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260: 436-438, 1976. PubMed: 1062678
22974: Ingleton PM, et al. Radiation induced osteogenic sarcoma in the rat as a model of hormone-responsive differentiated cancer. Lab. Anim. Sci. 27: 748-756, 1977. PubMed: 338978
22975: Atkins D, et al. Rat osteogenic sarcoma cells: isolation and effects of hormones on the production of cyclic AMP and cyclic GMP. Endocrinology 101: 555-562, 1977. PubMed: 195798
22989: Crawford A, et al. Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. Biochem. Biophys. Res. Commun. 82: 1195-1201, 1978. PubMed: 212039
23013: Gallinaro BJ, et al. Activation of protein kinase C inhibits ATP-induced [Ca2+]i elevation in rat osteoblastic cells: selective effects on P2Y and P2U signaling pathways. J. Cell. Physiol. 162: 305-314, 1995. PubMed: 7860638
23381: . . Clin. Orthop. Relat. Res. 140: 247-254, 1979.
23398: Manolagas SC, et al. 1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines. J. Biol. Chem. 255: 4414-4417, 1980. PubMed: 6929275
23491: Underwood JC, et al. Structural and functional correlations in parathyroid hormone responsive transplantable osteogenic sarcomas. Eur. J. Cancer 15: 1151-1158, 1979. PubMed: 294355
24392: . . Trans. Biochem. Sic. 6: 239-241, 1978.
26135: Partridge NC, et al. Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43: 4308-4315, 1983. PubMed: 6575864
26136: Martin TJ, et al. Hormonal influences on bone cells. Methods Enzymol. 145: 324-336, 1987. PubMed: 3474491
26137: Forrest SM, et al. Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif. Tissue Int. 37: 51-56, 1985. PubMed: 3922597
33152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651 |
|
