CRL-1746 ST 猪睾丸细胞系

ST (ATCC® CRL-1746?)
Organism  Sus scrofa, pig 
Tissue  testis
Product Format  frozen 
Morphology  fibroblast 
Culture Properties  adherent 
Biosafety Level  1 
Disease  Normal 
Age  80 to 90 days gestation 
Gender  male 
Applications  This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
Storage Conditions  liquid nitrogen vapor phase 
Clinical Data  male
Comments  This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
Complete Growth Medium  The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
 
Subculturing  Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions  Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor  SJ Wessman 
References  McClurkin AW, Norman JO. Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J. Comp. Med. Vet. Sci. 30: 190-198, 1966. PubMed: 4224292