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产品详情

  • 产品名称：CCL-138 Detroit 562 人咽头癌胸水转移细胞

  • 产品型号：Detroit 562
  • 产品厂商：美国标准生物品收藏中心（ATCC）
  • 产品价格：0
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简单介绍：
CCL-138 Detroit 562 人咽头癌胸水转移细胞 ,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件,
详情介绍：
CCL-138 Detroit 562 人咽头癌胸水转移细胞
Organism Homo sapiens, human
Tissue pharynx: derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease pharyngeal carcinoma
Age *****
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
  Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  
*****
Caucasian
female
  Genes Expressed  
keratin
  Virus Susceptibility   Human poliovirus 1 
Vesicular stomatitis virus               CCL-138 Detroit 562 人咽头癌胸水转移细胞
   Comments  
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
 
Subculturing

 

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.         CCL-138 Detroit 562 人咽头癌胸水转移细胞
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C              CCL-138 Detroit 562 人咽头癌胸水转移细胞
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